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KMID : 0363620080290010167
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Journal of Korean Oriental Medicine
2008 Volume.29 No. 1 p.167 ~ p.178
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The Effect of the Water Extract of Angelica Sinens on Gliosis Repression of Astrocyte after Hypoxic injury
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Lee Seung-Hee
Moon Seong-Jin
Shin Jin-Bong
Hae Rae-Kyoung
Seong Kee-Moon
Yang Jae-Hoon
Song Bong-Keun
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Abstract
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Object: Gliosis becomes a physical and mechanical barrier to axonal regeneration. Reactive gliosis induced by
hypoxic brain injury is involved with up-regulation of CD81 and GFAP. The current study was to examine the effect of the Angelica Sinens on CD81 and GFAP regulation after hypoxic brain injury in the astrocyte.
Methods: MTT assay was performed to examine cell viability, and cell based ELISA, western blot and PCR were
used to detect the expression of CD81 and GFAP.
Results: The following results were obtained: 1. Using ELISA, western blot and PCR from the astrocyte after hypoxic injury, CD81 and GFAP expression was seen to have increased. 2. After the administration of Angelica Sinens extract to astrocyte following hypoxic injury, CD81 and GFAP expression was down regulated significantly. The water extract of Angelica Sinens prevented cell destruction by hypoxic induced with CoCl2.
Conclusion: These results indicate that Angelica Sinens could suppress reactive gliosis, which disturbs astrocyte regeneration after hypoxic brain injury by controlling the expression of CD81 and GFAP.
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KEYWORD
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Angelica Sinens, gliosis, CD81, GFAP, hypoxic brain injury, astrocyte
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